|
KMID : 0613820210310070671
|
|
Journal of Life Science
2021 Volume.31 No. 7 p.671 ~ p.676
|
|
|
Lethal (2) Essential for Life [l(2)efl] Gene in the Two-spotted Cricket, Gryllus bimaculatus (Orthoptera: Gryllidae)
|
|
Kwon Ki-Sang
Lee Nu-Ri
Kwon O-Yu
|
|
|
Abstract
|
|
|
|
A cDNA encoding the protein lethal (2) essential for life [l(2)efl] was cloned from Gryllus bimaculatus and named GBl(2)efl. This protein is composed of 189 amino acids, including an N-glycosylation site and 15 phosphorylation sites. Its predicted molecular mass is 21.19 kDa, with a theoretical isoelectric point of 6.2. The secondary structure of GBl(2)efl was predicted from the identification of random coils (56.08%), alpha helices (22.22%), extended strands (17.99%), and beta turns (3.7%) through sequence analyses. A homology analysis revealed that GBl(2)efl exhibited a high similarity with other species at the amino acid level, ranging from 52% to 69%. While GBl(2)efl mRNA expression was higher in the dorsal longitudinal flight muscle following a three-day starvation and in the Malpighian tubules following a one-day starvation, no changes in expression were detected in other tissues. Furthermore, tunicamycin-induced endoplasmic reticulum (ER) stress resulted in an approximately 1.8-fold higher expression in the fat body compared with the wild type.
|
|
|
KEYWORD
|
|
|
Endoplasmic reticulum (ER) stress, G. bimaculatus l(2)efl [GBl(2)efl], lethal (2) essential for life (l(2)efl), two-spotted cricket Gryllus bimaculatus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|